Fig. 3
From: Long noncoding RNA HOTAIR promotes breast cancer development by targeting ZEB1 via sponging miR-601
HOTAIR directly interacted with miR-601 in BC. a The binding sites between HOTAIR-WT/MUT and miR-601 were obtained. b, c Dual-luciferase reporter assay was carried out to test the luciferase activity of HOTAIR-WT/MUT after cells were transfected with miR-601 mimic or miR-NC. d RIP assay results indicated that the enrichment of HOTAIR was increased in RIP-Ago2 of BC cells transfected with miR-601. e, f MiR-601 expression was remarkably downregulated in BC tissues and cells compared to adjacent normal tissues and MCF-10A cells, respectively. g The negative relation between HOTAIR and miR-601 was analyzed by Pearson correlation analysis. h The HOTAIR overexpression efficiency was detected by qRT-PCR. i The expression of miR-601 was measured by qRT-PCR to evaluate the effect of HOTAIR overexpression and knockdown on miR-601 expression. *P < 0.05