Table 2 Primers used for q-PCR analysis
From: EO771, the first luminal B mammary cancer cell line from C57BL/6 mice
Gene | Species | Size (bp) | RefSeq | Forward primer | Reverse primer |
|---|---|---|---|---|---|
Aromatase | Human | 207 | NM_000103.3 | 5′-TGGCAAGCTCTCCTCATCAA-3′ | 5′-TGTCAGGTCACCACGTTTCT-3′ |
Aromatase | Mouse | 142 | NM_007810.4 | 5′-GCTACGTGGATGTGTTGACC-3′ | 5′-TTGATGAGGAGAGCTTGCCA-3′ |
ERBB2 | Human | 172 | NM_004448.3 | 5′-TGTGTGGGAGCTGATGACTT-3′ | 5′-TCTTGGCCGACATTCAGAGT-3′ |
Erbb2 | Mouse | 152 | NM_001003817.1 | 5′-GGCTGCTGGACATTGATGAG-3′ | 5′-AAAGGTCATCAGCTCCCACA-3′ |
ERα | Human | 265 | NM_000125.3 | 5′-GTGCCTGGCTAGAGATCCTG-3′ | 5′-AGAGACTTCAGGGTGCTGGA-3′ |
Erα | Mouse | 231 | NM_007956.5 | 5′-ATTATGGGGTCTGGTCCTGC-3′ | 5′-CATCTCTCTGACGCTTGTGC-3′ |
ERβ | Human | 173 | NM_001040275.1 | 5′-AAGAAGATTCCCGGCTTTGT-3′ | 5′-TCTACGCATTTCCCCTCATC-3′ |
Erβ | Mouse | 202 | NM_207707.1 | 5′-CTTTGCTCCAGACCTCGTTC-3′ | 5′-AATCATGGCCTTCACACACA-3′ |
GAPDH | Human | 149 | NM_001289745.3 | 5′-TGACCACAGTCCATGCCATC-3′ | 5′-CAGCTCAGGGATGACCTTGC-3′ |
Gapdh | Mouse | 109 | NM_001289726.1 | 5′-ACCCCAGCAAGGACACTGAGCAAG-3′ | 5′-GGCCCCTCCTGTTATTATGGGGGT-3′ |
PR | Human | 224 | NM_001202474.3 | 5′-ATGGAAGGGCAGCACAACTA-3′ | 5′-AGGGCTTGGCTTTCATTTGG-3′ |
Pr | Mouse | 196 | NM_008829.2 | 5′-CAGCGCTTCTACCAACTCAC-3′ | 5′-TTTTGTGAAAGAGGAGCGGC-3′ |