Fig. 3

Circ-ITCH acted as a decoy for miR-106a in ovarian cancer cells. a The binding sites between miR-106a and circ-ITCH were predicted by StarBase v.2.0. b, c The relative luciferase activity in A2780 and OVCAR3 cells co-transfected with circ-ITCH-WT or circ-ITCH-MUT and miR-106a mimics (miR-106a) or miR-control (miR-con) was detected by dual-luciferase reporter assay. d, e Anti-Ago2 RIP assay was performed in A2780 and OVCAR3 cells after transfection with miR-106a or miR-con, and then the expression of circ-ITCH was examined by qRT-PCR. f MiR-106a expression in human ovarian epithelium cell line ISOE80 and ovarian cancer cell lines A2780 and OVCAR3 was detected by qRT-PCR. g, h The level of miR-106a in A2780 and OVCAR3 cells transfected with pcDNA, circ-ITCH, si-con (si-control) or si-circ-ITCH was examined by qRT-PCR. ***P < 0.001