Fig. 4

GATA3 inhibits Oxa resistance of CRC cells by miR-29b. a, b Viabilities of HT-29 and DLD1 cells in each group. The relative viability of cells normalized to the viability of cells from Scramble + Oxa group. The viability of over 100% meant that the viability was higher than that in Scramble + Oxa group. c-f Apoptosis and apoptosis rate of HT-29 and DLD1 cells in each group (g, h) GATA3 and Caspase3 + cleaved caspase3 protein levels in HT-29 and DLD1 cells. i, j MiR-29b mRNA levels in HT-29 and DLD1 cells. The Oxa concentration of HT-29 cells was set to 11 μmol/L; the Oxa concentration of DLD1 cells was set to 28 μmol/L. OE Over expression, Oxa Oxaliplatin, shRNA GATA3 shRNA, NC negative control, Agomir miR-29b agomir, Anagomir miR-29b antagomir. Significance of P values in a, d, h, i : vs. GATA3 scramble + Oxaliplatin, ^**p < 0.01; vs. GATA3 shRNA + Oxaliplatin, ^#p < 0.05, ^##p < 0.01; vs. miR-29b agomir NC + GATA3 shRNA + Oxaliplatin, ^{^}p < 0.05, ^{^^}p < 0.01. Significance of P values in a, d, h, i: vs. GATA3 scramble + Oxaliplatin, ^**p < 0.01; vs. GATA3 shRNA + Oxaliplatin, ^#p < 0.05, ^##p < 0.01; vs. miR-29b agomir NC + GATA3 shRNA + Oxaliplatin, ^{^}p < 0.05, ^{^^}p < 0.01