Fig. 4

Silenced miR-329-3p expression reversed partial function of si-KCNQ1OT1 on proliferation, migration, invasion, and apoptosis. a The expression of miR-329-3p was detected by qRT-PCR after transfected with anti-miR-329-3p or anti-miR-329-3p-control (con) into SW480 and LS1034 cells. b, c The proliferative ability was measured after SW480 and LS1034 cells were transfected with si-KCNQ1OT1 or si-KCNQ1OT1 + anti-miR-329-3p. d Cell apoptosis was assessed by staining with annexin V and propidium iodide 48 h. the percentage of apoptotic cells was determined using flow cytometric analysis. e, f The cells migration and invasion were performed by MTT assay after transfected with si-KCNQ1OT1 or si-KCNQ1OT1 + anti-miR-329-3p. g, h The protein levels of EMT markers (E-cadherin, MMP9), CyclinD1, apoptosis-associated proteins (Bcl-2, Cleaved-casp-3) were measured in SW480 or LS1034 cells by western blot analysis. *P < 0.05, vs. control group