Fig. 5

FOXM1 was the direct target of miR-159-5p. a Schematic representation of the potential binding sites of miR-149-5p with MIAT and FOXM1. b The luciferase activities were assessed after wild-type or mutant FOXM1 3′UTR luciferase reporter vector was co-transfected with miR-149-5p mimic or negative control mimic into A549 and H1299 cells. c The expression levels of FOXM1 in lung cancer cells and normal lung cells were measured by qRT-PCR. d Western blot assay shows FOXM1 expression in A549 cells and H1650 cells after transfected with miR-149-5p mimic or inhibitor. e FOXM1 was negatively correlated with miR-149-5p expression in NSCLC tissues. f Knockdown of MIAT decreased FOXM1 expression in A549 cells, and this effect was reversed by co-transfection of miR-149-5p inhibitor. g Overexpression of MIAT increased FOXM1 expression in H1650 cells, and this effect was reversed by co-transfection of miR-149-5p mimic. h FOXM1 was positively correlated with MIAT expression in NSCLC tissues. i, j Flow cytometric analysis of the cell cycle after MIAT knockdown in A549 and H1299 cells. ***p < 0.001