Fig. 6

RACK1 promotes CSCC cell apoptosis and suppresses tumor growth in vivo and in vitro by regulating the miR-302b/c/d-3p-CCNO axis. a The protein expression of Bcl-2, Cyclin D1, cleaved PARP, and cleaved caspase 3 in CSCC cell line (CasKi) detected by Western blot analysis, with protein bands assessed; b The cell cycle entry of CSCC cell line (CasKi) detected by flow cytometry; c The cell proliferation detected by EdU assay; d The cell apoptosis detected by TUNEL assay; e The tumors collected at 40 d after CasKi cell transplantation into nude mice; f Tumor volume in nude mice transplanted with CasKi cells overexpressing RACK1. g Tumor volume in nude mice transplanted with CasKi cells overexpressing RACK1. h The expression of miR-302 in mouse tumors detected by RT-qPCR; I, The protein levels of RACK1, CCNO, Bcl-2, CyclinD1, cleaved PARP, and cleaved caspase 3 in mouse tumors detected by Western blot analysis, with protein bands assessed. *p < 0.05 cells or mice treated with oe-NC. The values in the figure were measurement data, and expressed as mean ± standard deviation. Independent sample t test was used for the data comparison between two groups. Repeated measures ANOVA with Bonferroni post hoc test was used for data comparison among groups at different time points. The experiment was repeated 3 times independently. N = 05