Fig. 3

CircUBAP2 severs as a sponge of miR-361-3p and directly suppresses its expression in the cytoplasm in CC cells. a The predicted binding sequences between circUBAP2 and miR-361-3p was listed. b, c Luciferase reporter assay was performed in SiHa and HeLa cells co-transfected with WT-circUBAP2 or MUT-circUBAP2 and miR-361-3p mimic or mimic-NC. d The relative expression of miR-361-3p was evaluated by qRT-PCR after the biotinylated-circUBAP2 pull-down assays in SiHa and HeLa cells. e, g The expression of miR-361-3p was detected using qRT-PCR in CC tissues and cell lines. f The correlation between circUBAP2 and miR-361-3p expression was analyzed in CC patients. h The level of miR-361-3p was examined by qRT-PCR in SiHa and HeLa cells transfected with sh-circUBAP2. *P < 0.05