Fig. 2

TRG-AS1 adsorbs miR-4500 in the HCC cells. a, b Subcellular fractionation assay and FISH assay evaluated the location of TRG-AS1 in HCC cells. c RIP assay was performed using Ago2 antibody and RT-qPCR detected the enrichment of TRG-AS1 in both HCC cells. d The predicted targets for TRG-AS1 binding target via miRDB and starBase. e RT-qPCR examined miR-4500 expression in HCC cells and THLE-2 cells. f The enrichment of TRG-AS1 in both HCC cells was tested by RNA pull down assay under the condition of Bio-miR-4500-WT/Mut. g The binding sites of TRG-AS1 to miR-4500 were predicted via ENCORI. h The transfection effect of miR-4500 mimics was detected via RT-qPCR. i Effects of miR-4500 mimics on luciferase activity of reporter gene with wild-type or mutant TRG-AS1 were observed by luciferase reporter assay. **P < 0.01