Fig. 5
From: USP18 directly regulates Snail1 protein through ubiquitination pathway in colorectal cancer
USP18 regulated Snail1 expression through ubiquitination. a Correlation verification between USP18 and Snail1 mRNA in 60 colon cancer specimens. b The correlation between USP18 and Snail1 protein expression was calculated in 60 pairs of colon cancer specimens. c–d Western blot and qRT-PCR analysis the relationships between USP18 and Snail1. e Snail1 protein expression analysis with CQ and MG132 treatments. f Co-precipitation analysis of USP18 and Snail1 protein in SW480 cell. g Co-localization of immunofluorescence analysis of USP18 and Snail1 in SW480 and DLD1 cell. h Co-precipitation of USP18 and Snail1 in the 293T cell. i Ubiquitination testing of USP18 in DLD1 cell line. j Ubiquitination testing of USP18 in SW480 cell line. k, l Cycloheximide (protein degradation rate) analysis showed that USP18 could inhibit Snail1 protein degradation. *P < 0.05, **P < 0.01, ***P < 0.001