Fig. 5

MiR-590-5p targeted THBS2 in NSCLC cells. a The potential binding site between THBS2 3′UTR and miR-590-5p was predicted by the online software TargetScan. b Dual-luciferase reporter assay was used to measure the luciferase activity of THBS2-WT or THBS2-MUT reporter plasmid in A549 and H1299 cells transfected with miR-NC or miR-590-5p. c QRT-PCR was used to detect the mRNA expression of THBS2 in NSCLC cells. d The protein level of THBS2 in NSCLC cells was tested by Western blot. e The mRNA expression of THBS2 in A549 and H1299 cells transfected with si-circ_0020123-1 or si-circ_0020123-2 was detected by qRT-PCR. f Western blot was used to measure the protein level of THBS2 in A549 and H1299 cells transfected with si-circ_0020123-1 or si-circ_0020123-2. *P < 0.05