Fig. 6

MiR-326 exerted its role through SMAD6 in HCC cells. a Western blot analysis was conducted to assess the influence of SMAD6 overexpression on SMAD6 protein levels of miR-326-elevated HCCLM3 and Huh7 cells. b–f Impacts of SMAD6 augmentation on miR-326 enhancement-mediated proliferation, colony formation, cell cycle, migration, and invasion of HCCLM3 and Huh7 cells were determined with CCK-8 (b), colony formation (c), flow cytometry (d), wound healing (e), or transwell assays (f). g, h Effects of SMAD6 overexpression on the levels of Cyclin D1, MMP2, and MMP9 of miR-326-elevated HCCLM3 and Huh7 cells were evaluated via western blot analysis. *P < 0.05