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Fig. 3 | Cancer Cell International

Fig. 3

From: LncRNA DLX6-AS1 aggravates the development of ovarian cancer via modulating FHL2 by sponging miR-195-5p

Fig. 3

MiR-195-5p was a target of DLX6-AS1 and its expression was declined in OC tissues and cell lines. a The expression of miR-195-5p in OC tissues and adjacent healthy tissues was measured by qRT-PCR. b Spearman’s correlation analysis revealed the correlation between DLX6-AS1 level and miR-195-5p level in OC tissues. c The expression of miR-195-5p in IOSE80, SKOV3 and A2780 was measured by qRT-PCR. d Bioinformatics tool starBase analyzed the binding sites between DLX6-AS1 and miR-195-5p. e, f The relationship between DLX6-AS1 and miR-195-5p was confirmed by dual-luciferase reporter assay. g The interaction between DLX6-AS1 and miR-195-5p was further confirmed by RIP assay. (H) The change of miR-195-5p expression level was detected by qRT-PCR in SKOV3 and A2780 cells with DLX6-AS1 overexpression or knockdown. *P < 0.05

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