Fig. 5

FHL2, up-regulated in OC tissues and cell lines, was a target of miR-195-5p. a, b The expression of FHL2 at mRNA level and protein level in OC tissues and adjacent healthy tissues was measured by qRT-PCR and western blot, respectively. c, d Spearman’s correlation analysis revealed the correlation between FHL2 mRNA level and miR-195-5p level or DLX6-AS1 level in OC tissues. e The expression of FHL2 in IOSE80, SKOV3 and A2780 was measured by western blot at the protein level. f Bioinformatics tool starBase analyzed the binding sites between FHL2 3′ UTR and miR-195-5p. g, h The relationship between FHL2 and miR-195-5p was confirmed by dual-luciferase reporter assay. i The interaction between FHL2 and miR-195-5p was further confirmed by RIP assay. j The protein level of FHL2 was detected by western blot in SKOV3 and A2780 cells transfected with miR-195-5p, NC, miR-195-5p + pcDNA-DLX6-AS1 or miR-195-5p + pcDNA-NC. *P < 0.05