Fig. 2

TSA enhances Ac-α-tubulin upregulation and cell apoptosis induced by tanespimycin. a Calu-1 cells were treated with the given concentrations of tanespimycin (0, 0.5, 1.0 μM) or combination with TSA (200 nM) for 48 h, and then the cells were subjected to preparation of whole-cell protein lysates and the given proteins (Ac-α-tubulin, α-tubulin, HDAC6, PARP, LC3B-II and β-actin) were detected by Western blot analysis. b Calu-1 cells were seeded in 96-well plate and on the 2nd day treated with the given concentrations of tanespimycin (0, 0.25, 0.5, 1.0, 2.0 μM) or combination with TSA (200 nM) for 48 h. Cell number was estimated by SRB assay for calculation of cell survival. Points, mean of four replicate determinations; bars, SD