Fig. 4

Rapamycin inhibits cell apoptosis induced by tanespimycin. Calu-1 cells were treated with tanespimycin (0, 0.5, 1.0 μM) or combination with Rapamycin (50 nM) for 48 h, and then (a), the whole-cell protein lysates were prepared and the given proteins (Ac-α-tubulin, α-tubulin, LC3B-II, cleaved-Caspase-9, cleaved-Caspase-3 and β-actin) were detected by Western blot analysis. b The cells were subjected to take photos by inverted phase contrast microscope to observe the survival state