Fig. 5

3-MA down-regulates LC3B-II and promotes cell apoptosis induced by tanespimycin. Calu-1 cells were treated with tanespimycin (0, 0.5, 1.0 μM) or combination with 3-MA (1 mM) for 48 h; a the cells were subjected to preparation of whole-cell protein lysates and the given proteins (Ac-α-tubulin, α-tubulin, cleaved-Caspase-8, cleaved-Caspase-9, cleaved-PARP, LC3B-II and β-actin) were detected by Western blot analysis. b the cells were subjected to take photos by inverted phase contrast microscope to observe the apoptotic state