Fig. 6

Z-vad inhibits apoptosis and up-regulates α-tubulin acetylation induced by tanespimycin. Calu-1 cells were treated with the given concentrations of tanespimycin (0, 0.5, 1.0 μM) or combination with Z-vad (10 μM) for 48 h, and then the cells were subjected to preparation of whole-cell protein lysates and the given proteins (Ac-α-tubulin, cleaved-Caspase-8, cleaved-Caspase-3 and β-actin) were detected by Western blot analysis