Fig. 7

HDAC6 and TAT1 modulate the expression levels of α-tubulin acetylation in common. a Calu-1 cells were treated with 1.0 μM tanespimycin for the indicated time (0, 4, 8, 12, 24, 48 h). b Calu-1 cells were treated with the given concentrations of tanespimycin (0, 0.5, 1.0 μM) or combination with Docetaxel (0.5 nM) for 48 h. c, d Calu-1 cells were silenced by TAT1 siRNA and HDAC6 siRNA respectively, and then treated with 1.0 μM tanespimycin for 48 h. All the above cells were subjected to preparation of whole-cell protein lysates and the given proteins (a Ac-α-tubulin, HDAC6, TAT1 and β-actin; b Ac-α-tubulin, HDAC6 and β-actin; c HDAC6, Ac-α-tubulin and β-actin; d TAT1, Ac-α-tubulin and β-actin) were detected by Western blot analysis