Fig. 3

Effects of TAGLN overexpression on other genes and signaling pathways in RKO cells. Over-expression of TAGLN in RKO human colon cancer cells resulted in 184 genes differentially expressed with at least a twofold change (P < 0.05). a Functional enrichment (including cellular components, molecular functions, biological processes) and signaling pathway analysis of the DEGs were performed. b Topological analysis of the network illustrating the relationship between the proteins encoded by the DEGs. Genes in the red circle are core genes. The blue circle is the core module. The combinations of genes in the red and the blue circle are the key genes. c Signaling pathway enrichment analysis of the key genes identified the Rho signaling pathway. d Levels of the identified key genes as measured by cDNA microarray and real time RT-PCR. Relative gene expression was illustrated on a log-transformed scale. Data was obtained from three biological replicates