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Fig. 4 | Cancer Cell International

Fig. 4

From: OIP5-AS1 contributes to tumorigenesis in hepatocellular carcinoma by miR-300/YY1-activated WNT pathway

Fig. 4

LncRNA OIP5-AS1 sponges miR-300 and promotes cell growth in HCC. a The upstream lncRNAs of miR-300 were predicted via starBase. b The impact of miR-300 on above predicted lncRNAs in HCC cells were detected by luciferase reporter assay. c OIP5-AS1 expression in five HCC cell lines and normal THLE-3 cells was detected by qRT-PCR. d The existence of miR-300, OIP5-AS1 and YY1 in RISCs was confirmed by RIP assay. e The binding site between miR-300 and OIP5-AS1 was predicted by starBase. f The interplay between miR-300 and OIP5-AS1 was examined by luciferase reporter assay. g OIP5-AS1 expression was examined by qRT-PCR after sh-NC or sh-OIP5-AS1#1/2 were transfected into HepG2 and MHCC97H cells. hj The proliferation was assessed by CCK-8, colony formation and EdU assays in sh-OIP5-AS1#1 transfected HepG2 and MHCC97H cells. k Caspase-3 activity test and western blot analysis detected the influence of OIP5-AS1 silence on cell apoptosis and cell cycle in HepG2 and MHCC97H cells. **P < 0.01

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