Fig. 3

ANGPT2 is a target of miR-145-5p. a The expression levels of ANGPT2 mRNA in the miR-145-5p mimic and NC mimic groups are tested by qRT-PCR; b The protein expression levels of ANGPT2 in BGC-823 and AGS cells are examined by western blot; c Binding sites of miR-145-5p on ANGPT2 3′-UTR are predicted by a bioinformatics database; d Sequences of WT-ANGPT2 and MUT-ANGPT2; e Relative luciferase activity in BGC-823 and AGS cells transfected with either WT-ANGPT2 or MUT-ANGPT2 is assayed by dual-luciferase assay; *p < 0.05