Fig. 2

EIF3J-AS1 acts as a molecular sponge for miR-1343-3p in glioma. a, b Subcellular fractionation and FISH assays were combined to explore the location of EIF3J-AS1. c RNA pull down was performed to study the potential interaction between candidate miRNAs and EIF3J-AS1. d The expression of miR-1343-3p was detected in glioma cells and normal one. e RIP assay revealed that EIF3J-AS1 had the potential to bind to Ago2. f RNA pull down validated the interaction between EIF3J-AS1 and miR-1343-3p. g Putative and mutate miR-1343-3p binding sites with EIF3J-AS1 were displayed. h MiR-1343-3p expression was up-regulated by transfection of miR-1343-3p mimics. i Dual-luciferase reporters showed that miR-1343-3p mimics decreased the luciferase activity of vector containing EIF3J-AS1-WT. j, k Colony formation and EdU assays were performed to evaluate cell proliferation after up-regulating miR-1343-3p. l, m TUNEL and Caspase 3/8/9 activity assays were used to explore glioma cells apoptotic ability after up-regulating miR-1343-3p. **P < 0.01