Fig. 1

ING1b regulates p53-acetylation and downstream activation of pro-apoptotic pathway. a Relative protein expression of p53, acetylated p53 (at Lys382) and ING1b in OSCC cell lines YD-9 (wild type TP53), YD-8 (TP53 point mutation), and YD-38 (TP53 deletion). GAPDH was used as a loading control. Data is representative of three independent experiments. b Immunoblot analysis of total and acetylated p53 (at Lys382), and pro-apoptotic Bax and p21 in YD-9 and YD-8 cells stably transduced with control or INGb1 shRNA shows ING1b regulates acetylation and downstream expression of pro-apoptotic proteins. GAPDH was used as a loading control. Data is representative of three independent experiments. Numbers below the figure shows relative expression of ING1b as determined by densitometry analysis. c Cell proliferation was assayed for 3 days in YD-9 and YD-8 cells transiently transfected with control or ING1b shRNA, 48 h after transfection. Data represented in from 3 different independent experiments. Error bars, SD