Fig. 4

CircZFR directly interacted with miR-578 by binding to miR-578. a Venn diagrams representing the putative miRNAs that bind to circZFR identified by CircInteractome and CircBANK online algorithms. b, c The levels of miR-578, miR-944 and miR-532-3p by qRT-PCR in sh-NC-infected or sh-circZFR-transduced MCF7 and BT-549 cell lines. d qRT-PCR for miR-578 expression in the two BC cell lines transfected with miR-NC mimic or miR-578 mimic. e Schematic of the miR-578-binding sites within circZFR and the mutation of the seed sequence. f Relative luciferase activity in MCF7 and BT-549 cell lines cotransfected with circZFR-WT or circZFR-MUT and miR-NC mimic or miR-578 mimic. g qRT-PCR for circZFR level in cell lysates incubated with Bio-NC or Bio-miR-578 and streptavidin beads. *P < 0.05