Fig. 2From: Mitochondrion-mediated iron accumulation promotes carcinogenesis and Warburg effect through reactive oxygen species in osteosarcomaIron promotes osteosarcoma cell lines proliferation and affects cell cycle progression. a Cell viability assay of SAOS-2 and U2OS cells treated with 100 µM FAC, 100 µM DFO or PBS for 0–72 h. b Cell cycle analysis of SAOS-2 and U2OS cells by flow cytometry. c Plate colony formation assay of each group. d, e Representative immunofluorescence images and percentages of EdU-positive cells. f Iron increased PCNA expression while DFO showed an inverse result (*p < 0.05, **p < 0.01, mean ± SD, n = 3)Back to article page