Fig. 1

Up-regulation of miR-596 suppressed the proliferation of EOC cells by arresting cell cycle and stimulating cell apoptosis. a The enhanced expression of miR-596 in EOC cell lines (SKOV3, OVCAR3, A2780) compared to human ovarian surface epithelial cell line (HOSEpiC) was validated by qRT-PCR. b qRT-PCR confirmed the overexpression of miR-596 in A2780 and OVCAR3 cells by transfecting with miR-596 mimics. c, d The hindered proliferation of A2780 and OVCAR3 cells under miR-596 elevation was indicated by CCK-8 and colony formation assays. e, f Flow cytometry analyses evidenced that cell cycle was arrested at G0/G1 phases and apoptosis rate was increased under miR-596 upregulation. g TUNEL staining assay detected that the percent of TUNEL-positive cells was augmented in miR-596 mimics group relative to miR-NC group. Scale bar = 50 μm. h Western blot analyzed that miR-596 enhancement fortified the level of Bax and cleaved PARP and reduced the level of Bcl2. **P < 0.01