Fig. 2

EP300 transcriptionally stimulated miR-596 to restrain the growth of EOC cells. a qRT-PCR and western blot verified that EP300 expression in A2780 and OVCAR3 cells was silenced by shEP300 but elevated by pcDNA3.1/EP300 (EP300). b Dual-luciferase reporter assay detected that the luciferase activity of miR-596 promoter was decreased or increased after EP300 was inhibited or overexpressed. c ChIP assay validated that the high H3K27ac status in miR-596 promoter in EOC cells compared with that in normal HOSEpiC cells. d EP300 expression at mRNA and protein levels was reduced in EOC cells relative to HOSEpiC cells, as assessed by qRT-PCR and western blot. e qRT-PCR confirmed that miR-596 level was inhibited or enhanced in EOC cells in response to EP300 depletion or overexpression, separately. f qRT-PCR proved the successful inhibition of miR-596 in EOC cells by miR-596 inhibitor. g Cell viability was controlled by EP300 upregulation but recovered after miR-496 suppression, as monitored by CCK-8 assay. h, i Flow cytometry analyses indicated that EP300 induced cell cycle arrest and apoptosis, while such effects were offset by inhibited miR-596. j TUNEL assay results proved that EP300 augmented the percentage of TUNEL-positive cells, while inhibiting miR-596 could counteract such influence. Scale bar = 50 μm. **P < 0.01