Fig. 4

BRD4 or KPNA4 knockdown served restraining effects on the growth of EOC cells. a The transfection of shBRD4 suppressed BRD4 expression at mRNA and protein levels in A2780 and OVCAR3 cells, as detected via qRT-PCR and western blot. b–g The results of functional assays demonstrated that depleted BRD4 impaired EOC cell proliferation, blocked cell cycle progression, and facilitated cell apoptosis. h As examined by qRT-PCR and western blot, the expression of KPNA4 was weakened by shKPNA4 compared to that in the control group (shNC). i–n The suppressive impact of KPNA4 knockdown on the growth of A2780 and OVCAR3 cells was assessed by CCK-8, colony formation and TUNEL assays and flow cytometry and western blot analyses. **P < 0.01