Fig. 5

MiR-596 regulated cell proliferation, cell cycle and apoptosis in EOC via targeting BRD4 and KPNA4. a, b Elevation on the mRNA and protein levels of BRD4 or KPNA4 severally induced by transfection of pcDNA3.1/BRD4 (BRD4) or pcDNA3.1/KPNA4 (KPNA4) in A2780 cells was determined by qRT-PCR and western blot. c, d The results of CCK-8 and colony formation assays proved that miR-596 upregulation-restrained cell proliferation was reversed by overexpressing BRD4 or KPNA4. e–h The normalization of BRD4 or KPNA4 overexpression on cell cycle and apoptosis of miR-596-enhanced A2780 cells were confirmed by flow cytometry, TUNEL staining (Scale bar = 50 μm) and western blot. **P < 0.01