Fig. 3
From: Development of syngeneic murine cell lines for use in immunocompetent orthotopic lung cancer models
Validation of a KrasG12D.Tgfbr2−/− cell line. a H&E stain showing orthotopic tumor formation by line X911. Scale bar is 100 µm and the red line denotes the tumor border. b PCR showing the recombinant KrasG12D allele (305 bp) and Tgfbr2 allele (220 bp) in X911 cells but not in CMT or LLC cells. c Western blot showing KRASG12D expression and TGFBR2 loss in X911 cells. The band at ~ 70 kD is nonspecific (NS)