Fig. 5
From: Development of syngeneic murine cell lines for use in immunocompetent orthotopic lung cancer models
Validation of a KrasG12D.Pten+/−.p53+/−.GFP+ cell line. a H&E stain showing orthotopic tumor formation by line X381. Scale bar is 100 µm and the red line denotes the tumor border. b PCR of genomic DNA showing recombination of the KrasG12D allele (305 bp), Pten allele (300 bp), and Tp53 allele (612 bp) in X381 cells. c Western blot showing expression of KRASG12D and loss of PTEN and TP53 in X381 cells. The absence of detectable PTEN and TP53 expression by Western blot suggests loss of the remaining wild type alleles. Both CMT and LLC cells harbor Tp53 mutations (Howard Li, personal communication). d X381 cells express GFP; scale bar is 50 µm