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Table 1 Primer sequences and PCR reaction conditions for BM742401

From: Epigenetic silencing of long non-coding RNA BM742401 in multiple myeloma: impact on prognosis and myeloma dissemination

Primer set Forward primer (5′–3′) Reverse primer (5′–3′) MgCl2/Tm/cycles References
(I) BM742401 MSP
 M-MSP CGT TTA GGT AGA TAA TGA GAG TCG C AAA TCA AAC GTT CTA TAA CCT CCG 1.5 mM/60 °C/38X [28]
 U-MSP TGT GTT GTT TAG GTA GAT AAT GAG AGT TGT CCA AAT CAA ACA TTC TAT AAC CTC CA 2.0 mM/60 °C/38X
(II) qRT-PCR
 BM742401 TTG GTT CTT TTC TAC AAG GAT GTC CGA ATC GGT CAA TGT CCA CC NA NA
 GAPDH ACC ACA GTC CAT GCC ATC ACT TCC ACC ACC CTG TTG CTG TA NA NA
  1. Tm, annealing temperature; M-MSP, methylated MSP; U-MSP, unmethylated MSP
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