Table 1 Primer sequences and PCR reaction conditions for BM742401
Primer set | Forward primer (5′–3′) | Reverse primer (5′–3′) | MgCl2/Tm/cycles | References |
|---|---|---|---|---|
(I) BM742401 MSP | ||||
 M-MSP | CGT TTA GGT AGA TAA TGA GAG TCG C | AAA TCA AAC GTT CTA TAA CCT CCG | 1.5 mM/60 °C/38X | [28] |
 U-MSP | TGT GTT GTT TAG GTA GAT AAT GAG AGT TGT | CCA AAT CAA ACA TTC TAT AAC CTC CA | 2.0 mM/60 °C/38X | |
(II) qRT-PCR | ||||
 BM742401 | TTG GTT CTT TTC TAC AAG GAT GTC | CGA ATC GGT CAA TGT CCA CC | NA | NA |
 GAPDH | ACC ACA GTC CAT GCC ATC ACT | TCC ACC ACC CTG TTG CTG TA | NA | NA |