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Fig. 4 | Cancer Cell International

Fig. 4

From: Long noncoding RNA MIR99AHG promotes gastric cancer progression by inducing EMT and inhibiting apoptosis via miR577/FOXP1 axis

Fig. 4

MIR99AHG/miR-577/FOXP1 axis regulated the malignant phenotype of GC cells. a mRNA level of FOXP1 was confirmed by qPCR in BGC-823 and SGC-7901 cell following MIR99AHG knockdown, MIR99AHG overexpression, and MIR99AHG overexpression plus FOXP1 knockdown. b Protein expression level of FOXP1 was confirmed by western blot in BGC-823 and SGC-7901 cell following MIR99AHG knockdown, MIR99AHG overexpression, and MIR99AHG overexpression plus FOXP1 knockdown. c, d CCK8 proliferation assay in BGC-823 and SGC-7901 cells after MIR99AHG overexpression and MIR99AHG overexpression plus FOXP1 knockdown. e Trans-well assay shows invasion effect. f, g Qualification of the trans-well assay. h E-cadherin and N-cadherin expression levels were detected by Western blotting. *p < 0.05, **p < 0.01 and ***p < 0.001 (two-way ANOVA)

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Cancer Cell International

ISSN: 1475-2867

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