Table 1 Oligonucleotides used in this study
Gene | Sequence (5′ To 3′) | Product (bp) | Application |
|---|---|---|---|
LINE-1 expression primers (GenBank accession number L19088)Â (25) | F: TGAGAACGGGCAGACAGACT R: AGGTCTGTTGGAATACCCTGCC | 129 | RT-qPCR |
LINE-1 AQAMA Primers | F: GGGTTTATTTTATTAGGGAGTGTTAGA R: TCACCCCTTTCTTTAACTCAAA | 148 | qPCR |
LINE-1 AQAMA methylation specific probe | FAM-TGCGCGAGTCGAAGT-BHQ1 | – | qPCR |
LINE-1 AQAMA unmethylation specific probe | HEX-TGTGTGAGTTGAAGTAGGG-BHQ1 | – | qPCR |