Fig. 3

Investigation the MH-NDV combined therapy to induce apoptosis in treated cells using acridine orange and propidium iodide: (a–d) AMJ13 cancer cells indicated that MH-NDV induces apoptosis as evidenced red-stained cells, Untreated control cells emitting green fluorescence. e–h MCF-7 cells were showing that the number of apoptotic cells is higher in the combination treatment. i–l There was no effect against REF cells by combination therapy using MH-NDV. j, k MH-NDV treated AMJ13, and MCF-7 breast cancer cells expressed significant apoptotic cell death in comparison to monotherapies and the untreated control cells. m no significant changes in all treatment modalities. Values represent the (mean ± SD). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. The magnification of all images was 400×