Fig. 4

MH–NDV combination efficiently inhibits glycolysis products in the treated breast cancer cells but not in normal cells in comparison to monotherapies. a MH–NDV combined therapy induces a significant decrease in the activity of hexokinase in AMJ13 and MCF-7 cancer cell lines. At the same time, there is no significant reduction in normal REF cells (b, c) measurement of pyruvate and ATP levels in cancer cells significantly reduced in MH-NDV combination therapy in comparison to both monotherapies and untreated control cells. However, the reduction in the normal REF cells was not significant. d Measurements of pH levels in AMJ13 and MCF-7 breast cancer cells indicate that MH-NDV treated cells supernatant was more alkaline in comparison to both monotherapies significantly, while untreated control cells showing acidity as well as the control and treated non-cancerous REF cells. All data shown are mean ± SEM (*P < 0.05) data from three different experiments using unpaired t-test, stars on MH and NDV column means it is significant in compare to each other and star on the com means it is significant in compare to monotherapies (MH and NDV). Star on the control column means it is significant when compared to MH, NDV and Com