Table 1 Sequences used for qRT-PCR and siRNA assays
From: circRAE1 promotes colorectal cancer cell migration and invasion by modulating miR-338-3p/TYRO3 axis
Names | Sequences (5′— 3′) | |
|---|---|---|
circRAE1 | Forward | AGAGAGGGGCCTGATTGTCTA |
| Â | Reverse | AGTGTGCATCTGCTGATGTTTC |
E-cadherin | Forward | GCTGGACCGAGAGAGTTTCC |
Reverse | CAAAATCCAAGCCCGTGGTG | |
Vimentin | Forward | GGACCAGCTAACCAACGACA |
Reverse | AAGGTCAAGACGTGCCAGAG | |
Linear RAE1 | Forward | AGGCAGTACTTCTCCAGGTTCA |
Reverse | GGCAAGGTTGGTGGGCTAAA | |
TYRO3 | FORWARD | AGTTGGCTGTGGACCCTGGAG |
REVERSE | AGGATGTGCGGCTGTGAGGAG | |
GAPDH | Forward | ATGGGGAAGGTGAAGGTCG |
Reverse | TTACTCCTTGGAGGCCATGTG | |
miR-338-3p | Forward | CGCGTCCAGCATCAGTGATT |
Reverse | AGTGCAGGGTCCGAGGTATT | |
RT Primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAACAA | |
U6 | Forward | CTCGCTTCGGCAGCACATATACT |
Reverse | ACGCTTCACGAATTTGCGTGTC | |
RT Primer | AAAATATGGAACGCTTCACGAATTTG | |
si-circRAE1-Homo1 | Sense | GGAUCAAAGCUCCAAACUACA |
Antisense | UAGUUUGGAGCUUUGAUCCAA | |
si-circRAE1-Homo2 | Sense | GCAGUGCAACUACAGACAAUC |
Antisense | UUGUCUGUAGUUGCACUGCCA | |
si-circRAE1-Homo3 | Sense | GGACCUCAGCAGUAACCAAGC |
Antisense | UUGGUUACUGCUGAGGUCCCA | |
si-TYRO3 | Sense | GGUGGAGAGGAACUACGAAGA |
| Â | Antisense | UUCGUAGUUCCUCUCCACCAG |
Negtive control | Sense | AUGGAAUUUUGUUGUGAAAUC |
Antisense | UUCACAACUUAAUUCCAAGAU | |