Fig. 5

NONO and SFPQ promotes ACLY mRNA stability in HCC cells. a RIP analysis of the interactions between IGF2BP1 and ACLY mRNA in MHCC97H cells transfected with si-NONO for 24 h, and then stimulated with DEN for 24 h. b qRT-PCR analysis of nuclear and cytoplasmic ACLY mRNA expression in MHCC97H cells transfected with si-NONO for 24 h, and then stimulated with DEN for 24 h. c RIP analysis of the interactions between NONO and ACLY mRNA in MHCC97H cells transfected with si-IGF2BP1 for 24 h, and then stimulated with DEN for 24 h. d qRT-PCR analysis of nuclear and cytoplasmic ACLY mRNA expression in MHCC97H cells transfected with si-IGF2BP1 for 24 h, and then stimulated with DEN for 24 h. e COIP analysis of the interactions of NONO and SFPQ in DEN-stimulated MHCC97H cells. f RIP analysis of the interactions between NONO and ACLY mRNA in MHCC97H cells transfected with si-SFPQ for 24 h, and then stimulated with DEN for 24 h. g COIP analysis of the interactions of MYC-NONO and FLAG-SFPQ in DEN-stimulated MHCC97H cells. h RIP analysis of the interactions between MYC-NONO or NONO truncations and ACLY mRNA in MHCC97H cells. i COIP analysis of the interacting domain of NONO with FLAG-SFPQ in MHCC97H cells. Data are represented as mean ± SD (n = 3; *p < 0.05)