Fig. 4

Interaction between circRNA_104889 and miR4458. a Schematic representation of two miRNA binding sites (labeled blue arc and red base) in circRNA_104889, while the 3′UTR region of caspase-3 harbors a putative miR4458 binding site, which is highly conserved across multiple species. b The change of miR4458 expression after downregulating circRNA_104889 with siRNA2. c Dual-luciferase reporter assays using reporter constructs with the entire circRNA_104889 sequence, then HEK293 cells were co-transfected with pmiR-RB-REPORT™ (WT) and either miR4458 or control mimics. d Cell invasion assay. Data are presented as mean ± SD from 3 independent experiments in triplicates, n = 3. Significant effect of the treatments, ** p < 0.01, *** p < 0.001