Fig. 14

miR-221-3p inhibits RUNX2 expression in PC cells. a Direct binding sequences of miR-221-3p and RUNX2 mRNA. b–e Luciferase activities of 3′UTR RUNX2-luc constructs in PC cells after transfection with mimic, inhibitor or control. The results were assessed with t tests (unpaired). f, g RUNX2 expression after transfection with mimic, inhibitor or control determined by RT-qPCR. h–k RUNX2 expression after transfection with mimic, inhibitor or control determined by western blotting