Fig. 2

Radiation treatment increased UCA1 expression and EIF4G1 protein level in PCa cells. a QRT-PCR analysis was conducted to detect UCA1 expression in PCa cell lines (22RV1 and DU145 cells) and human normal prostatic epithelial cells RWPE1. b, c The mRNA (b) or protein (c) level of EIF4G1 was determined by qRT-PCR or western blot in 22RV1 and DU145 cells, respectively. d, e UCA1 expression was detected in 22RV1 (d) and DU145 (e) cells every 3 h after 0 or 6 Gy radiation treatment. f, g UCA1 expression was measured in 22RV1 (f) and DU145 (g) cells after 0, 2, 4, 6 Gy radiation treatment for 24 h. h EIF4G1 protein level was upregulated after 22RV1 and DU145 cells treated with 6 Gy radiation for 24 h. ^*P < 0.05