Fig. 5

Upregulation of EIF4G1 weakened the effect of UCA1 knockdown on the radiosensitivity of PCa cells. 22RV1 and DU145 cells were transfected with si-UCA1 or co-transfected with si-UCA1 and pcDNA-EIF4G1. a, b The mRNA (a) and protein (b) expression of EIF4G1 were measured in PCa cells. c, d Cell proliferation was detected using MTT assay at the indicated times after 6 Gy irradiation treatment. e, f The clonogenic survival curve was established at day 14 after transfected cells received indicated Gy irradiation. g Cell apoptosis was measured by flow cytometry after radiotherapy. h, i Protein expression of Cyclin D1, Bcl-2, and Bax was detected in 22RV1 (h) and DU145 (i) cells exposure to radiotherapy. ^*P < 0.05