Fig. 1
From: XBP1 negatively regulates CENPF expression via recruiting ATF6α to the promoter during ER stress
CENPF expression is downregulated in osteosarcoma U2OS cells in response to ER stress. a U2OS cells were treated with DMSO vehicle (control), BFA (1 μg/ml), TG (1 μM) or TM (2.5 μg/ml) for 0, 6, or 24 h. The CENPF mRNA levels were quantified by real-time reverse transcription-PCR (q-RT-PCR) and normalized to 18S RNA. b Changes in CENPF protein levels induced by BFA, TG and TM treatment in U2OS cells. CENPF and α-tubulin levels were determined by western blot analysis. Data are representative of 3 independent experiments. Data are presented as mean ± s.e.m. *P < 0.05, **P < 0.005, ***P < 0.001, ****P < 0.0001