Fig. 2

Downregulation of circ_0000285 suppressed cell progression in OS. a The expression of circ_0000285 and HIPK3 was measured in si-NC, si-circ_0000285-1 and si-circ_0000285-2 groups. b CCK-8 assay was used to detect cell viability in si-NC and si-circ_0000285 groups in SJSA1 and U2OS cells. c Transwell migration and invasion assays were used to assess cell migration and invasion in si-NC and si-circ_0000285 groups in SJSA1 and U2OS cells. d Colony formation assay was applied to assess cell proliferation in si-NC and si-circ_0000285 groups in SJSA1 and U2OS cells. e, f Flow cytometry was used to detect cell cycle and cell apoptosis in si-NC and si-circ_0000285 groups in SJSA1 and U2OS cells. *P < 0.05