Fig. 3

The expressions of BZW2 were negatively regulated by miR-4500. a Schematic diagram showed predicted binding sites between wild-type or mutated miR-4500 and the 3′UTR of BZW2. b, c Luciferase reporter was used to determine the relative luciferase activities in cells that were transfected with plasmids containing BZW2 3′UTR-wide type (wt) or mutant (mt) and miR-4500 mimics or miR-NC. d Biotin-coupled miRNA pulldown assay was used to determine enrichment of BZW2 mRNAs in the RNA-RNA complexes. e, f qRT-PCR and western blotting were used to determine the mRNA and protein levels of BZW2 in each group. The data represent the mean ± SD. *P < 0.05, **P < 0.01, ns indicates no significance