Fig. 4

LINC00174 competed with the BZW2 for binding with miR-4500. a Schematic diagram showed predicted binding sites between wild-type or mutated LINC00174 and miR-4500. b, c Luciferase reporter was used to determine the relative luciferase activities in cells that were transfected with plasmids containing LINC00174-wt or mt and miR-4500 or miR-NC. d qRT-PCR was used to determine relative enrichment of LINC00174 RNAs in the RNA-RNA complexes. e, f qRT-PCR was used to determine the mRNA levels of genes including LINC00174, miR-4500, and BZW2 in LP cells that were transfected with LINC00174 siRNAs (si-LINC00174-1 and si-LINC00174-2) or si-NC and in the LP cells that were transfected with LINC00174 overexpression plasmids (LINC00174-OE) or empty plasmid vector (VEC). g The protein expressions of BZW2 were determined using western blot in LINC00174 knockdown or LINC00174 overexpressed cells. The data represented are the means ± S.D. *P < 0.05, **P < 0.01, ns indicates no significance