Fig. 3

circ_0028171 acts as a sponge for miR-218-5p in osteosarcoma cells. a, b qRT-PCR was conducted to detect six miRNA candidates in MG-63 and 143B cells pulled down by circ_0028171 probe. c, d Relative luciferase intensity were detected in OS cells after co-transfecting with circ_0028171 ’UTR-wild type and miRNA mimics. e The change circ_0028171expression obviously influenced miR-218-5p expression in MG-63 and 143B cells. f miR-218-5p expression level in 20 paired OS tissues. g miR-218-5p and circ_0028171 expression was tested by Pearson’s correlation. h The miR-218-5p-binding sites in circ_0028171 sequence was predicted by bioinformation. i The relative luciferase activity was detected after co-transfection with circ_0028171 reporter plasmid and miR-218-5p in MG-63 and 143B cells. j, k The anti-AGO2 RIP assay was performed to detect the connection between miR-218-5p and circ_0028171