Fig. 1

LOXL1-AS1 aggravates laryngocarcinoma cell proliferation, migration and EMT. a RT-qPCR analysis detected the overexpressed LOXL1-AS1in laryngocarcinoma cell lines (SNU-899, SNU-46, AMC-HN-8, Tu-177 and M4E) compared to normal pharyngal epithelial cell line (NP69). b The knockdown efficiency of LOXL1-AS1 was examined using RT-qPCR after transfecting three shRNAs targeting LOXL1-AS1 into Tu-177 and M4E cells. c, d CCK8 and colony formation assays detected the hindered cell proliferation in response to LOXL1-AS1 silencing. e Wound healing assay demonstrated the suppression on migration ability induced by LOXL1-AS1 silencing in Tu-177 and M4E cells. Relative wound width was calculated using the relative value of wound widths at 24 h to that at 0 h. f Transwell assay determined the hinder migration of Tu-177 and M4E cells with LOXL1-AS1 inhibition. G. IF examined the elevated expression of E-cadherin and the reduced level of N-cadherin in Tu-177 and M4E cells under LOXL1-AS1 inhibition. The standard deviation of control groups in a and b was calculated by 2^−ΔΔCt method as follow: ΔCt (Control) = ΔCt (target, Control) – ΔCt (reference, Control); mean ΔCt (Control) = {ΔCt−1 (Control) + ΔCt−2 (Control) + ΔCt−3 (Control)}/3; ΔΔCt (Control) = ΔCt (Control) − mean ΔCt (Control). *p < 0.05, **p < 0.01