Fig. 5

ERRFI1 expression is down-regulated by miR-148a-3p from U251 cells-derived exosomes to activate EGFR/MAPK signaling pathway, thereby enhancing tube formation ability of HUVECs. a Expression of miR-148a-3p and ERRFI1 in HUVECs was determined by RT-qPCR in the co-culture system of U251 cells-derived exosomes (U251-exo) and oe-NC/oe-ERRFI1-transiently transfected HUVECs (U6 and GAPDH were used as internal controls, respectively). b Western blot analysis of ERRFI1, ratios of p-EGFR/EGFR and p-ERK1/2/ERK1/2, and extent of ERK1/2/ERK1/2 phosphorylation in the co-culture system of U251-exo and oe-ERRFI1-transiently transfected HUVECs. c CCK-8 assay to evaluate the proliferation of oe-ERRFI1-transiently transfected HUVECs co-cultured with U251-exo. d Lumen formation ability of oe-ERRFI1-transiently transfected HUVECs co-cultured with U251-exo. e Expression of VEGF and VEGFR2 was tested using ELISA in cell supernatant in the co-culture system of oe-ERRFI1-transiently transfected HUVECs and U251-exo. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 vs. the treatment with PBS + oe-NC or with U251-exo + oe-NC. U251 mentioned in this figure refers to U251-MG. Data are shown as mean ± SEM. The cell experiments were performed in 3 repeats and each repeat was performed in technical replicate (triplicate). Repeated measures ANOVA was used for data analysis among multiple groups at different time points. One-way ANOVA was used for multi-group comparison with Tukey's test